David Grayless<p>Cross-linking and <a href="https://mastodon.social/tags/immunoprecipitation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>immunoprecipitation</span></a> (CLIP, or CLIP-seq) is a method used in <a href="https://mastodon.social/tags/molecularBiology" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>molecularBiology</span></a> that combines UV <a href="https://mastodon.social/tags/crosslinking" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>crosslinking</span></a> with immunoprecipitation in order to identify <a href="https://mastodon.social/tags/RNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>RNA</span></a> binding sites of <a href="https://mastodon.social/tags/proteins" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>proteins</span></a> on a transcriptome-wide scale, thereby increasing our understanding of post-transcriptional regulatory networks. CLIP can be used either with antibodies against endogenous proteins, or with common peptide tags (including FLAG, V5, HA, and others) or affinity purification.</p>