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David Grayless<p>Cross-linking and <a href="https://mastodon.social/tags/immunoprecipitation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>immunoprecipitation</span></a> (CLIP, or CLIP-seq) is a method used in <a href="https://mastodon.social/tags/molecularBiology" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>molecularBiology</span></a> that combines UV <a href="https://mastodon.social/tags/crosslinking" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>crosslinking</span></a> with immunoprecipitation in order to identify <a href="https://mastodon.social/tags/RNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>RNA</span></a> binding sites of <a href="https://mastodon.social/tags/proteins" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>proteins</span></a> on a transcriptome-wide scale, thereby increasing our understanding of post-transcriptional regulatory networks. CLIP can be used either with antibodies against endogenous proteins, or with common peptide tags (including FLAG, V5, HA, and others) or affinity purification.</p>
Daniel Conderman<p>Let’s bring back the personal web! Build your own site, cross-link with others, and revive the golden age of the internet. <a href="https://open.substack.com/pub/danielconderman/p/bring-back-the-webmaster-how-personal?r=bd3r5&amp;utm_campaign=post&amp;utm_medium=web" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">open.substack.com/pub/danielco</span><span class="invisible">nderman/p/bring-back-the-webmaster-how-personal?r=bd3r5&amp;utm_campaign=post&amp;utm_medium=web</span></a> 🌐 <a href="https://mastodon.social/tags/Webmaster" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Webmaster</span></a> <a href="https://mastodon.social/tags/BringBackTheWeb" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>BringBackTheWeb</span></a> <a href="https://mastodon.social/tags/WWW" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>WWW</span></a> <a href="https://mastodon.social/tags/PersonalSites" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>PersonalSites</span></a> <a href="https://mastodon.social/tags/CreativeWeb" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>CreativeWeb</span></a> <a href="https://mastodon.social/tags/OldSchoolInternet" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>OldSchoolInternet</span></a> <a href="https://mastodon.social/tags/RetroWeb" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>RetroWeb</span></a> <a href="https://mastodon.social/tags/DIYWeb" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>DIYWeb</span></a> <a href="https://mastodon.social/tags/InternetNostalgia" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>InternetNostalgia</span></a> <a href="https://mastodon.social/tags/CrossLinking" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>CrossLinking</span></a></p>
guyinahat<p>I just got a gallon of <a href="https://mastodon.social/tags/metricide" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>metricide</span></a>. It contains like 2% <a href="https://mastodon.social/tags/glutaraldehyde" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>glutaraldehyde</span></a>. It's supposed to be the most potent <a href="https://mastodon.social/tags/crosslinking" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>crosslinking</span></a> agent for <a href="https://mastodon.social/tags/gelatin" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>gelatin</span></a> in <a href="https://mastodon.social/tags/carbontransfer" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>carbontransfer</span></a>. I'm hoping that this fixes my luck with this <a href="https://mastodon.social/tags/photography" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>photography</span></a> <a href="https://mastodon.social/tags/process" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>process</span></a>. I'm kind of <a href="https://mastodon.social/tags/procrastinating" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>procrastinating</span></a> on this. I've failed like 3 times already and I'm afraid of success I guess. But maybe it's time to break the cycle. How will I know it's working? I'm gonna mix black color into a sample and then try to dissolve it in <a href="https://mastodon.social/tags/water" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>water</span></a>.</p>
JIPB<p>This one's phragmo...tastic?!<br>Du et al. describe a novel <a href="https://mstdn.science/tags/microtubule" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>microtubule</span></a> and <a href="https://mstdn.science/tags/actin" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>actin</span></a> filament <a href="https://mstdn.science/tags/crosslinking" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>crosslinking</span></a> protein that stabilizes the <a href="https://mstdn.science/tags/phragmoplast" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>phragmoplast</span></a> during <a href="https://mstdn.science/tags/cytokinesis" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>cytokinesis</span></a>.<br><a href="https://doi.org/10.1111/jipb.13497" rel="nofollow noopener" target="_blank"><span class="invisible">https://</span><span class="">doi.org/10.1111/jipb.13497</span><span class="invisible"></span></a> <br>@wileyplantsci<br> <a href="https://mstdn.science/tags/JIPB" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>JIPB</span></a> <a href="https://mstdn.science/tags/PlantScience" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>PlantScience</span></a> <a href="https://mstdn.science/tags/cell" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>cell</span></a> <a href="https://mstdn.science/tags/division" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>division</span></a> <a href="https://mstdn.science/tags/Arabidopsis" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Arabidopsis</span></a></p>